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exp2  (Nobilis Therapeutics)

 
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    Nobilis Therapeutics exp2
    Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
    Exp2, supplied by Nobilis Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/exp2/pmc12722989-78-9-15?v=Nobilis+Therapeutics
    Average 86 stars, based on 1 article reviews
    exp2 - by Bioz Stars, 2026-08
    86/100 stars

    Images

    1) Product Images from "Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus"

    Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

    Journal: Poultry Science

    doi: 10.1016/j.psj.2025.106178

    Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; EXP2: NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
    Figure Legend Snippet: Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; EXP2: NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).

    Techniques Used: Virus, Control

    Relative mRNA expression (2⁻ΔΔCt) of innate immune-related genes in chickens from EX 2–4 vaccinated with different live viral vaccines. (A) EXP2 (NDV, trachea); (B) EXP3 (IBDV, bursa of Fabricius); (C) EXP4 (IBV, trachea). Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). Bars connected by * or ** denote significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each gene, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; MDA5, melanoma differentiation-associated gene 5; NF-κB, nuclear factor κB; IL-1β, interleukin 1β; IFN-β, interferon β; OAS, 2′−5′-oligoadenylate synthetase; PKR, protein kinase R; EO, essential oil.
    Figure Legend Snippet: Relative mRNA expression (2⁻ΔΔCt) of innate immune-related genes in chickens from EX 2–4 vaccinated with different live viral vaccines. (A) EXP2 (NDV, trachea); (B) EXP3 (IBDV, bursa of Fabricius); (C) EXP4 (IBV, trachea). Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). Bars connected by * or ** denote significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each gene, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; MDA5, melanoma differentiation-associated gene 5; NF-κB, nuclear factor κB; IL-1β, interleukin 1β; IFN-β, interferon β; OAS, 2′−5′-oligoadenylate synthetase; PKR, protein kinase R; EO, essential oil.

    Techniques Used: Expressing, Vaccines, Comparison

    Antibody titers against NDV, IBDV, and IBV in chickens from the four experimental groups. (A) EXP1 (non-vaccinated birds), (B) EXP2–4, birds vaccinated with NDV, IBDV, or IBV, respectively. Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). NDV antibodies were measured by hemagglutination inhibition (HI) and expressed as log₂ HI titers. IBDV and IBV antibodies were quantified by ELISA and expressed as sample-to-positive (S/P) ratios. Bars connected by * or ** indicate significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each virus, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. NDV, Newcastle disease virus; IBDV, infectious bursal disease virus; IBV, infectious bronchitis virus; EO, essential oil.
    Figure Legend Snippet: Antibody titers against NDV, IBDV, and IBV in chickens from the four experimental groups. (A) EXP1 (non-vaccinated birds), (B) EXP2–4, birds vaccinated with NDV, IBDV, or IBV, respectively. Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). NDV antibodies were measured by hemagglutination inhibition (HI) and expressed as log₂ HI titers. IBDV and IBV antibodies were quantified by ELISA and expressed as sample-to-positive (S/P) ratios. Bars connected by * or ** indicate significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each virus, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. NDV, Newcastle disease virus; IBDV, infectious bursal disease virus; IBV, infectious bronchitis virus; EO, essential oil.

    Techniques Used: HI Assay, Enzyme-linked Immunosorbent Assay, Virus, Comparison



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    Image Search Results


    Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; EXP2: NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).

    Journal: Poultry Science

    Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

    doi: 10.1016/j.psj.2025.106178

    Figure Lengend Snippet: Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; EXP2: NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).

    Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

    Techniques: Virus, Control

    Relative mRNA expression (2⁻ΔΔCt) of innate immune-related genes in chickens from EX 2–4 vaccinated with different live viral vaccines. (A) EXP2 (NDV, trachea); (B) EXP3 (IBDV, bursa of Fabricius); (C) EXP4 (IBV, trachea). Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). Bars connected by * or ** denote significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each gene, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; MDA5, melanoma differentiation-associated gene 5; NF-κB, nuclear factor κB; IL-1β, interleukin 1β; IFN-β, interferon β; OAS, 2′−5′-oligoadenylate synthetase; PKR, protein kinase R; EO, essential oil.

    Journal: Poultry Science

    Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

    doi: 10.1016/j.psj.2025.106178

    Figure Lengend Snippet: Relative mRNA expression (2⁻ΔΔCt) of innate immune-related genes in chickens from EX 2–4 vaccinated with different live viral vaccines. (A) EXP2 (NDV, trachea); (B) EXP3 (IBDV, bursa of Fabricius); (C) EXP4 (IBV, trachea). Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). Bars connected by * or ** denote significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each gene, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; MDA5, melanoma differentiation-associated gene 5; NF-κB, nuclear factor κB; IL-1β, interleukin 1β; IFN-β, interferon β; OAS, 2′−5′-oligoadenylate synthetase; PKR, protein kinase R; EO, essential oil.

    Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

    Techniques: Expressing, Vaccines, Comparison

    Antibody titers against NDV, IBDV, and IBV in chickens from the four experimental groups. (A) EXP1 (non-vaccinated birds), (B) EXP2–4, birds vaccinated with NDV, IBDV, or IBV, respectively. Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). NDV antibodies were measured by hemagglutination inhibition (HI) and expressed as log₂ HI titers. IBDV and IBV antibodies were quantified by ELISA and expressed as sample-to-positive (S/P) ratios. Bars connected by * or ** indicate significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each virus, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. NDV, Newcastle disease virus; IBDV, infectious bursal disease virus; IBV, infectious bronchitis virus; EO, essential oil.

    Journal: Poultry Science

    Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

    doi: 10.1016/j.psj.2025.106178

    Figure Lengend Snippet: Antibody titers against NDV, IBDV, and IBV in chickens from the four experimental groups. (A) EXP1 (non-vaccinated birds), (B) EXP2–4, birds vaccinated with NDV, IBDV, or IBV, respectively. Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). NDV antibodies were measured by hemagglutination inhibition (HI) and expressed as log₂ HI titers. IBDV and IBV antibodies were quantified by ELISA and expressed as sample-to-positive (S/P) ratios. Bars connected by * or ** indicate significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each virus, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. NDV, Newcastle disease virus; IBDV, infectious bursal disease virus; IBV, infectious bronchitis virus; EO, essential oil.

    Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

    Techniques: HI Assay, Enzyme-linked Immunosorbent Assay, Virus, Comparison